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Animal Cell Culture Freshney Free Ebook Download

Culture of Animal Cells: A Manual of Basic Technique and Specialized Applications, Seventh Edition provides the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. This text is an indispensable resource for those in or entering the field, including academic research scientists, clinical and biopharmaceutical researchers, undergraduate and graduate students, cell and molecular biology and genetics lab managers, trainees and technicians.

Animal Cell Culture Freshney Free Ebook Download

This new edition of Animal Cell Culture covers new or updated chapters on cell authentication, serum-free culture, apoptosis assays, FISH, genetic modification, scale-up, stem cell assays, 3-dimensional culture, tissue engineering and cytotoxicity assays. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. Everyone working in biological and medical research, whether in academia or a commercial organization, practising cell culture will benefit greatly from this book.

Introduction to basic principles Scaling-up of cell cultures Cell line preservation and characterization Development of serum-free media Three dimension culture Tissue engineering Cytotoxicity and viability assays Fluorescence in situ hybridization (FISH) Genetic modification Epithelial stem cell identification, isolation and culture Apoptosis assays

Cell culture is the most widely studied method in the field of life sciences. This term is generally used for the removal of cells, tissues or organs from a plant or an animal and their subsequent transfer into an artificial environment supporting their survival. The primary requirements for the optimum growth of cells comprise of regulated temperature, suitable growth medium, substrate for cell attachment and incubator that regulates pH and osmolality. The most crucial stage in cell culture is selecting the suitable growth medium. Cell culture media is the relevant energy source in cell culture that constitutes balance of amino acids, glucose, inorganic salts and serum as a foundation of hormones, growth factors and attachment factors. On the basis of requirement of different cell lines, media is divided into four types: balanced salt solutions (BSS), basal media, complex media and serum-free media. This chapter outlines the media and buffer components and preparation essential for maintaining the cell culture.

Therefore, it is important to ensure that the water that is employed in cell cultures is microorganisms-free, particularly from inorganic ions (heavy metals such as zinc, lead, etc.) endotoxins and other organic compounds (pesticides, humic acids, tannins, etc.).


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